Poor chromatographic peak shapes on Acquity - WKB54306
Article number: 54306
SYMPTOMS
- Poor chromatography - Peak Tailing
- Failing QC samples
- Decreasing peak response
- Leak test fails with hex cap on column inlet fitting
- Reproducibility issues
ENVIRONMENT
- ACQUITY UPLC I-Class (Plus and Bio)
- ACQUITY UPLC H-Class (Plus and Bio)
- ACQUITY UPLC Column Manager
- ACQUITY UPLC Column Heater
CAUSE
- Void in flow path
- An improperly fitted column in the column manager
Finger-tight column fittings that were tightened using tools may be damaged. The two-piece metal ferrule between the finger-tight fittings may have been pushed out of position.
- Improperly fitted tubing/loop in the flow path
FIX or WORKAROUND
- Refit the column/tubing/loop
- Replacing the PEEK ferrules if worn. (700003114 - Ferrule (PEEK), 1/16, 10/pkg)
- Replace the APH or post-column tubing if it is damaged due to over-tightening with tools. (205001757 - ACQUITY I-Class APH, SS, 12.5LG - PLUS and 700008868 - ASSY, WELDED TUBE, SS, .003 ID X 19.0 LG)
ADDITIONAL INFORMATION
PEEK ferrules should be replaced after every ten to twenty column changes.
APH of a different length may be required for some column manager configurations.
id54306, A-30HCM, Active Preheater, UPBINARY, UPCHA, UPCHAIVD, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPPCHA, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO