How to adjust the seals on an Alliance - WKB285739
OBJECTIVE or GOAL
Adjust the seals on an Alliance.
ENVIRONMENT
- 2695 Separations Module
- Alliance HPLC System
PROCEDURE
Before you run the function, confirm these things:
The sample management system is purged at least twice.
• The solvent is degassed 100% methanol (or another compatible solvent).
• You removed the column to avoid damaging it with a pressure pulse of approximately 6894 kPa (69 bar, 1000 psi).
• You installed on the red outlet line a restrictor that generates at least 6894 kPa (69 bar, 1000 psi) of back pressure at < 3.0 mL/min.
Tips:
• To attain sufficient back pressure without a column, you can partially cap the red line with a union and fitting plug assembly. Collect solvent runoff in an appropriate waste container.
• High-resistance restrictors (> 13788 kPa (138 bar, 2000 psi) @ 1 mL/min) can cause this test to take longer, and result in a missing restrictor error.
To adjust the seals:
1. On the Diagnostics screen, press the Adjust Seals screen key.
2. On the Adjust Seals dialog box, press the Start screen key.
At the end of the test, the screen displays this information:
• Pass or fail status
• Current and previous seal data
Confirm the seal data values—top, bottom, and width—fall within the acceptable ranges shown inthe tablebelow.
A seal geometry error can appear if these conditions apply:
• The adjust seals parameter values are not in the acceptable range.
• Air bubbles occur in the sample management system. (If so, purge the sample management system and try again.)
• Solvent is not properly degassed (even though air bubbles are not visible).
For more information, see 2695 Operator Guide
