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Waters

High back pressure with ACQUITY I-Class FTN - WKB27154

Article number: 27154

SYMPTOMS

  • Error: system over pressure
  • Higher than normal system pressure
  • Normal pressure returns when injector is in LOAD position

ENVIRONMENT

  • ACQUITY UPLC I-Class
  • ACQUITY UPLC
  • ACQUITY UPLC Sample Manager
  • Sample Manager-FTN

CAUSE

  • Extension loop installed
  • Injection volume greater than 15 µL
  • Flow rate less than 0.2 mL/min
  • Large sample volumes and low flow rate increase risk of blocking the standard 0.003 SST Seal Port

FIX or WORKAROUND

  1. Install needle assembly (700005279 - 30 µL, FTN).
  2. Install seal port (700010726 - SST, 0.007).

ADDITIONAL INFORMATION

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