How to perform UPLC SM-FL leak tests - WKB263857

OBJECTIVE or GOAL

Perform all UPLC SM-FL leak tests for customers.

ENVIRONMENT

• ACQUITY UPLC I-Class
• ACQUITY UPLC
• ACQUITY UPLC I-Class PLUS
• nanoACQUITY UPLC
• ACQUITY UPLC Sample Manager 
• ACQUITY UPLC M-Class

PROCEDURE

1. Open the console and navigate to the sample manager using the tree on the left-hand side.
2. From the drop-down menu control, select Prime Syringes.
3. Use 10% ACN in both weak and strong wash lines.
   • Select Wash and Sample syringes
   • Choose 6 Cycles
4. From the maintain drop-down menu:
   1. Select characterize > Needle seal
      • Run the test, wait for result, collect information (picture or snip tool works well)
   2. Select Syringe leak test...
      1. Select Sample Syringe
         • Run the test, wait for result, collect information
      2. Select Wash Syringe
         • Run the test, wait for result, collect information
      3. Select Needle seal
         • Run the test, wait for result, collect information
   3. Select Back pressure regulator test
      • Run the test, wait for result, collect information

ADDITIONAL INFORMATION

• A combination of aqueous and methanol or ACN for weak wash and/or 100% organic for strong wash will work as well
• Results are recorded under the console log, which can be accessed at a later time