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How to autozero the flow controllers on an ACQUITY M-Class with breaking fluidics connections (the best way) - WKB25522

Article number: 25522

OBJECTIVE or GOAL

Use the best method to autozero the flow controllers on an ACQUITY M-Class.

ENVIRONMENT

  • ACQUITY M-Class
  • ICS 1.53 or 1.55
  • Firmware 1.56
  • For ICS 1.74 and firmware 1.56, see the update at end of article

PROCEDURE

  1. Break the fluidics connection (if not already done) from the exit of the μBSM and remove the tubing.
  2. Prime the A and B lines of the μBSM for five minutes.
  3. Set the μBSM to flow at 25 μL/min 50:50 A/B for 15 minutes.

Note: Place an absorbent material below the outlet of the pump to capture the flowing solvent.

  1. Stop flow.
  2. Wait for at least five minutes and monitor the measured flow rate for stability.
  3. Pin plug the outlet of the μBSM.

     Pin plugs: finger tight pin plug.PNG     or Nano peek plug 2.PNG

  1. Again, wait for at least five minutes and monitor measured flow rate for stability.
  2. Run the autozero flow transducers diagnostic, from the Troubleshooting menu of each pump within the console.
  3. Reconnect tubing and ensure that there are no leaks.

 

Update for DP 2019 and newer (cf. release notes: https://www.waters.com/waters/support.htm?lid=135041575&lcid=135041574&type=RLSN)

A wizard has been introduced to ease up autozeroing the flow controllers; follow the wizard accordingly. The wizard flushes the solvents into the flow controller with up to 18 µL/min 50%A 50%B through the trapping flow path.

Autozero for "BSM only" system configuration selection will only show once Service Mode is enabled.

 

ADDITIONAL INFORMATION

 

id25522, UPMASM, UPMBSM, UPMSMFL

 

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