No peaks and poor chromatography on an H-Class system - WKB24767
Article number: 24767
SYMPTOMS
- No peak for caffeine sample
- No peaks running the gradient test
- Pressure trace does not change with gradient changes for AB and CD
- Pump and system leak tests fail
- Peaks returned with GPV replacement show tailing and lower sensitivity
- Peaks missing again after I2V check valve replacement and primary head rebuild
- Peaks return with removal of sample loop
- Wash of system with phosphoric acid shifts retention times
ENVIRONMENT
- ACQUITY UPLC H-Class
CAUSE
Injection valve leaks to waste during injections.
FIX or WORKAROUND
Replace the injection valve cartridge (700005236).
- See section 3.12 in ACQUITY UPLC SM-FTN Overview and Maintenance Information (715005051)
ADDITIONAL INFORMATION
id24767, A-30SDM, A-30SM, checkvalve, gradient proportioning valve, UPAQSM, UPISMFTN, UPPFTN, UPPQSM, UPQSM, UPQSM, UPQSM+, UPQSM+, UPQSMBIO, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN, UPSMFTN+, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO