Peak fronting observed on early eluting peaks with ACQUITY UPLC H-Class - WKB240989
Article number: 240989
SYMPTOMS
- Peak fronting observed on early eluting peaks with ACQUITY UPLC H-Class
- All system diagnostics, including dynamic leak test and needle seal readiness test, pass
- Later eluting peaks show no distortion
ENVIRONMENT
- ACQUITY UPLC H-Class
- Empower
CAUSE
The formic acid used as an additive in the mobile phase is old (possible formic acid degradation leading to insufficient acid concentration).
FIX or WORKAROUND
Prepare fresh mobile phases with a new, previously unopened batch of LCMS-grade formic acid.
ADDITIONAL INFORMATION
