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Waters

Carryover of standard sample in blank injections on ACQUITY H-Class Bio - WKB23379

Article number: 23379

SYMPTOMS

ENVIRONMENT

  • ACQUITY H-Class Bio
  • ACQUITY ELS Detector
  • ACQUITY PDA

CAUSE

Likely void volume in column outlet and flow cell inlet fittings and tubing interconnections

  • Systematic contamination was observed (after all the lines were changed) as carryover was finally flushed out.

FIX or WORKAROUND

  1. Replumb the PDA with a new PEEK line from the column outlet to the PDA flow cell inlet.
    • Any tubing after the injector that is not properly seated could retain contamination or void and be a source of carryover.

ADDITIONAL INFORMATION

 

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