Carryover of standard sample in blank injections on ACQUITY H-Class Bio - WKB23379
Article number: 23379
SYMPTOMS
- Carryover of polysorbate standard in test blank injections
- System flushed per recommendations in the Controlling Contamination document 715001307
ENVIRONMENT
- ACQUITY H-Class Bio
- ACQUITY ELS Detector
- ACQUITY PDA
CAUSE
Likely void volume in column outlet and flow cell inlet fittings and tubing interconnections
- Systematic contamination was observed (after all the lines were changed) as carryover was finally flushed out.
FIX or WORKAROUND
- Replumb the PDA with a new PEEK line from the column outlet to the PDA flow cell inlet.
- Any tubing after the injector that is not properly seated could retain contamination or void and be a source of carryover.
ADDITIONAL INFORMATION
id23379, A-30PDA, UPAPDA, UPARPPDA, UPBSMIVD, UPC2PDA, UPELS, UPELSARC, UPIBSMIVD, UPLCCOLHTR, UPNBINARY, UPPDA, UPPDAARC, UPPDAARCB, UPPDA-E, UPPDA-L, UPPDALTC, UPPDATC, UPPPDA, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR