Split peaks and contamination peaks in blanks - WKB224123

SYMPTOMS

• Split/broad peaks on samples   
• Peaks on blanks
• Replaced/investigated all possible parts/areas of contamination on LC modules (BSM, SM, CM/CH, column and MS)

ENVIRONMENT

• ACQUITY UPLC   
• I-Class
• H-Class

CAUSE

Contaminated mobile phases

FIX or WORKAROUND

1. Make up fresh mobile phase using new solvents and additives where possible.
2. Ensure that you are not using contaminated source of additive and/or solvent.

ADDITIONAL INFORMATION

To identify solvents as the source of the contamination, use a union between the pump outlet tubing and the column inlet tubing. 

Replace one component at a time (solvent, buffers, acid, etc.) to pinpoint the source of contamination.

Contamination of the solvents should appear as high background and a chromatographic peak.