With Empower 3 and ACQUITY UPLC PDA Detector, the spectrum shape is significantly different from the spectrum acquired by other instruments? - WKB205242

SYMPTOMS

• The spectrum shape is significantly different compared to the spectrum shape of the same compound acquired by other PDA detectors

ENVIRONMENT

• Empower 3
• ACQUITY UPLC PDA Detector
• ACQUITY UPLC PDA eλ Detector

CAUSE

• The resolution setting of the instrument method is different
• The check boxes of "Interpolate the 2nd order filter region" and "Interpolate 656 nm" in the instrument method are OFF
• The PDA detector cell is dirty

FIX or WORKAROUND

1. Check the resolution setting of the instrument method.
   → Is it equivalent to the value used for acquisition of the spectrum being compared?
2. Check if the "Interpolate the 2nd order filter region" and "Interpolate 656 nm" options of the instrument method are checked.
   → If it is OFF, turn it ON and run the measurement, and then compare the shapes.
3. Clean PDA detector cells.
   → If the cause is dirt on the cell, see if cleaning can help.
   ■ General cleaning
   ① Remove the column and connect to the detector with a union.
   ② Flush all mobile phase lines with 100% water at a flow rate of 0.2 mL/min for half a day.
   ③ Flush all mobile phase lines with 100% IPA at a flow rate of 0.2 mL/min for half a day.
   ④ Flush all mobile phase lines with 100% water at a flow rate of 0.2 mL/min for one hour.
   ⑤ Flush all mobile phase lines with 1% formic acid in water at a flow rate of 0.2 mL/min for three hours.
   ⑥ Flush all mobile phase lines with 2% ammonia in water at a flow rate of 0.2 mL/min for three hours.
   ⑦ Flush all mobile phase lines with 100% water at a flow rate of 0.2 mL/min for three hours.
   *Use wet prime when replacing the solvent.
   * In addition to the solvents listed above, if there is a solvent that is likely to easily dissolve the potential contamination (mainly the sample), flush with that solvent.
   * The specified time is a reference.

ADDITIONAL INFORMATION