How to run the manual Calibration Diagnostic on the 2475 Multi-Wavelength Fluorescence Detector - WKB193307

Article number: 193307

OBJECTIVE or GOAL

To manually perform the Calibration Diagnostic Test on the 2475 Multi-Wavelength Fluorescence Detector.

Manual wavelength calibration

You can calibrate the detector manually from the keypad by pressing the

manual calibration key at any time during detector operation or when

calibration errors arise during startup. You need not restart the detector after

a successful wavelength calibration.

Tip: Before you pump solvent or mobile phase through the system, flush the

lines with filtered, degassed, and sparged HPLC-grade water and continue to

pump at 1 mL/min.

To manually calibrate the wavelength:

Wavelength calibration message

The detector cycles through the calibration procedure and momentarily

displays a series of initialization messages similar to those displayed at

startup. If calibration is successful, the detector beeps three times.

If the maximum error is greater than 2.0 nm, the detector displays the

maximum error of the farthest calibration shift from the previous

calibration.

Calibration successful message

Press Enter. A “Calibration Complete” message appears momentarily before the home screen reappears. Other messages such as “Optimizing system performance” and “Restoring Last Setup” can appear before the home screen reappears.

You can review this procedure in the How to replace the flow cell on a 2475 Multi-Wavelength Fluorescence Detector video on Waters.com (starting at 2 minutes)

If calibration is not successful, repeat it.
If calibration is still not successful, shut down the detector, flush the lines with filtered, degassed HPLC grade water and continue to pump at 1 mL/min, cycle the power to restart the detector and let it initialize normally and repeat if necessary.
Run the detector normalization diagnostic test.
Requirement: If this test yields a failing result, repeat it.

id193307, 2475, 2475B, A-10FL, eluent