Skip to main content
Waters

How to flush a clogged HPLC flow cell - WKB1930

Article number: 1930

OBJECTIVE or GOAL

Unclog an HPLC flow cell

ENVIRONMENT

  • 2487 Dual-Wavelength Absorbance Detector
  • 996/2996 PDA Detector
  • 2489 UV/Vis Detector
  • 2998 Photodiode Array Detector
  • 2414 Refractive Index Detector

PROCEDURE

  1. Prepare a suitable solvent or 6M nitric acid solution.
  2. Remove the solvent filter and prime the system.
  3. Replace the column with a union.
  4. Reverse the flow cell inlet and outlet lines.
  5. Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating (1000 psi for UV, 100 psi for RI) for the flow cell.
  6. Flush at a low flow for at least one hour.
  7. Flush with HPLC grade water until the effluent returns to neutral.

ADDITIONAL INFORMATION

 


Not able to find a solution? Click here to request help.