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What might cause peaks to shift retention times when the instrument is running a gradient separation? - WKB191904

Article number: 191904

SYMPTOMS

  • Peaks shift retention times when the instrument is running a gradient separation

ENVIRONMENT

  • Gradient separation

CAUSE

Loss of prime or inadequate solvent delivery through at least one of the gradient proportioning lines.

FIX or WORKAROUND

  1. Prime and flush lines independently to establish proper flow and pressure. Make sure that solvents are prepared properly.

ADDITIONAL INFORMATION

 

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