Skip to main content
Waters

PDA baseline drifts during the organic portion of the gradient - WKB17475

Article number: 17475

SYMPTOMS

  • PDA baseline drifts upward during the organic portion of the gradient 
  • Lamp was replaced
  • Mobile phase was made two months ago
  • Solvent A: 99% water/1% ACN/0.1% TFA
  • Solvent B: 99% ACN/1% water/0.1% TFA

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • ACQUITY UPLC PDA Detector

CAUSE

Bad mobile phase

FIX or WORKAROUND

  1. Replace the mobile phase with freshly made solvent.
  2. Prime the system thoroughly with the new mobile phase.

ADDITIONAL INFORMATION

 

Not able to find a solution? Click here to request help.