Running an analysis on an H-Class yields longer retention times than it does on an I-Class - WKB16115
Article number: 16115
SYMPTOMS
- Running an analysis on an H-Class yields longer retention times than it does when performed on an I-Class
ENVIRONMENT
- ACQUITY H-Class UPLC System
- ACQUITY I-Class UPLC System
- ACQUITY Classic UPLC System
- ACQUITY Premier UPLC system
CAUSE
Larger dwell volume of the H-Class compared with the I-Class
FIX or WORKAROUND
This is expected because the dwell volume of the I-Class is 0.073 mL and the dwell volume of the H-Class is 0.375 mL.
Starting the gradient ahead of injection by a volume or time equal to the difference in dwell volume between the two systems can compensate for the different dwell volumes and address the differences in retention times.
ADDITIONAL INFORMATION
id16115, UPBSMIVD, UPIBSM, UPIBSM+, UPIBSMIVD, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPLCCOLHTR, UPNBINARY, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR