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Running an analysis on an H-Class yields longer retention times than it does on an I-Class - WKB16115

Article number: 16115

SYMPTOMS

  • Running an analysis on an H-Class yields longer retention times than it does when performed on an I-Class

ENVIRONMENT

  • ACQUITY H-Class UPLC System
  • ACQUITY I-Class UPLC System
  • ACQUITY Classic UPLC System
  • ACQUITY Premier UPLC system

CAUSE

Larger dwell volume of the H-Class compared with the I-Class

FIX or WORKAROUND

This is expected because the dwell volume of the I-Class is 0.073 mL and the dwell volume of the H-Class is 0.375 mL.

Starting the gradient ahead of injection by a volume or time equal to the difference in dwell volume between the two systems can compensate for the different dwell volumes and address the differences in retention times.

ADDITIONAL INFORMATION

Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer (720005723EN)

id16115, UPBSMIVD, UPIBSM, UPIBSM+, UPIBSMIVD, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPLCCOLHTR, UPNBINARY, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR

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