Running an analysis on an H-Class yields longer retention times than it does on an I-Class - WKB16115
Article number: 16115
SYMPTOMS
- Running an analysis on an H-Class yields longer retention times than it does when performed on an I-Class
ENVIRONMENT
- ACQUITY H-Class UPLC System
- ACQUITY I-Class UPLC System
- ACQUITY Classic UPLC System
- ACQUITY Premier UPLC system
CAUSE
Larger dwell volume of the H-Class compared with the I-Class
FIX or WORKAROUND
This is expected because the dwell volume of the I-Class is 0.073 mL and the dwell volume of the H-Class is 0.375 mL.
Starting the gradient ahead of injection by a volume or time equal to the difference in dwell volume between the two systems can compensate for the different dwell volumes and address the differences in retention times.