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How to make a sample loop from a length of tubing - WKB1551

Article number: 1551

OBJECTIVE or GOAL

Determine the length of tubing required to make a sample loop, based on the ID of the tubing.

ENVIRONMENT

  • HPLC instruments with a sample loop

PROCEDURE

  1. Determine the volume of the loop you want to create.
  2. Determine the ID of the tubing that you want to use to create the loop.
  3. Based on the list below, calculate the length of tubing you will cut to achieve the required volume.
    • Tube-cutting is recommended only for HPLC.
    • UPLC and nano/micro tubing should be cut at the factory.
  4. The volume of a loop is calculated by the inner diameter and the length of the tubing (µL/inch).
    • .0025 = .080 µL/inch
    • .005 = .322 µL/inch
    • .009 = 1.04 µL/inch
    • .020 = 5.148 µL/inch
    • .030 = 11.583 µL/inch
    • .040 = 20.592 µL/inch

Note: You must use the specific fittings required for the instrument.

ADDITIONAL INFORMATION

 


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