- Pressure is stable when running isocratic
- Baseline is good initially and through the first eight minutes of the gradient
- Flow rate is accurate from both A and B pumps
- Breeze 2
- 1525 Binary HPLC pump
- 2489 UV/Visible Detector
- 2707 Autosampler
Mixer is dirty
FIX or WORKAROUND
- Remove and sonicate the gradient mixer in methanol for 15 minutes.
If cleaning the mixer does not resolve the issue, you may need to replace the gradient mixer (WAT055847).