What are the maximum injection volumes for different injection types with a 2707 autosampler? - WKB12788

ENVIRONMENT

• Breeze 2
• 2707 Autosampler

ANSWER

Assuming that the 2707 contains the standard equipment (500-µL syringe, 1000-µL buffer tubing, 15-µL needle, and 100-µL sample loop), the following are the maximum injection volumes:

Full Loop - 100 µL

Partial Loop Needle Overfill - 0 to 50 µL

Partial Loop - 0 to 27 µL

ADDITIONAL INFORMATION

Formulas:

Full Loop = Loop Volume

Partial Loop Needle Overfill = Half the Loop Volume

Partial Loop =  (loop volume - (3 * needle volume)) / 2