No chromatographic peaks - WKB115238

SYMPTOMS

• No chromatographic peaks in the expected time window
• No signal
• Peaks visible when acquiring from time 0

ENVIRONMENT

• ACQUITY UPLC
• Alliance HPLC System

CAUSE

Both solvent bottles (A and B) contain organic solvent or both solvent bottles (A and B) contain water or (A and B) lines swapped.

FIX or WORKAROUND

Ensure to use the correct mobile phases for the selected solvent lines.

ADDITIONAL INFORMATION