How much sample is consumed using the partial loop with needle overfill injection mode on the ACQUITY UPLC Sample Manager? - WKB11277
Article number: 11277
ENVIRONMENT
- ACQUITY UPLC Sample Manager
- Partial loop with needle overfill (PLNO) injection mode
- ACQUITY I-Class Sample Manager - Fixed Loop
- Injection Mode: Partial loop with needle overfill (PLNO)
ANSWER
The specified injection volume plus 15.0 µL (14.0-µL pre-sample volume and 1.0-µL post sample volume) when using the default setting of "automatic".
The default overfill volume changes with respect to the needle configured:
- Advanced setting selected as "automatic"
- 10 ul needle (7.0µL pre-sample volume and 1.0µL post sample volume)
- 20 ul needle (10.0µL pre-sample volume and 1.0µL post sample volume)
ADDITIONAL INFORMATION
To confirm or change this, go to the Sample Manager Instrument Method in Empower or the Sample Manager Inlet Method in MassLynx and click Advanced.
ACQUITY UPLC Sample Manager Injection Mode Quick Reference Card.
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