- High-pressure shutdown due to blocked gradient mixer
- 1525 pump
Gradient mixer is blocked.
High-pressure shutdown occurs even with the reference waste valve open.
FIX or WORKAROUND
- Disconnect the inlet and outlet gradient mixer tubing connections.
- Connect the inlet tubing to the outlet port of the mixer and back flush at 0.1 mL/min in order to flush out the blockage slowly.
- If the blockage is buffer precipitate, you may need to flush with hot water instead of room temperature water.