Low sensitivity or no peaks at certain points - WKB101611

SYMPTOMS

Low sensitivity
No peaks
Incorrect retention time
Strange baseline

ENVIRONMENT

Alliance HPLC System
2695 Separations Module
2695D Dissolution Separations Module
2795 Separations Module
Alliance Series Column Heater
Alliance Series Column Heater/Cooler
ACQUITY APC
ACQUITY APC Isocratic Solvent Manager
ACQUITY APC Sample Manager - FTN
ACQUITY Arc
ACQUITY Arc Sample Manager FTN-R
ACQUITY UPLC
ACQUITY UPLC BSM (example, use other modules as needed)
ACQUITY UPLC Column Manager
ACQUITY UPLC H-Class
ACQUITY UPLC H-Class Bio
ACQUITY UPLC H-Class Quaternary Solvent Manager (QSM)
ACQUITY UPLC I-Class
ACQUITY UPLC H-Class PLUS
ACQUITY UPLC H-Class PLUS Bio
ACQUITY UPLC I-Class PLUS
ACQUITY UPLC M-Class
nanoACQUITY UPLC
PATROL UPLC Process Analysis System
ACQUITY UPLC Process Sample Manager
ACQUITY UPLC Column Heater
ACQUITY UPLC PDA Detector
ACQUITY UPLC Sample Manager
ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
ACQUITY UPLC Sample Organizer
ACQUITY UPLC Tunable UV Detector
ACQUITY UPLC FLR Detector
ACQUITY UPLC RI Detector
ACQUITY UPLC Online SPE Man
510 HPLC pump
515 HPLC Pump
600 MultiSolvent Delivery Pump
717plus Autosampler
1500 Series Column Heater
1515 Isocratic HPLC Pump
1525 Binary HPLC Pump
1525 Micro Binary HPLC Pump
2707 Sample Manager
2777 Sample Manager
e-SAT/IN Module
Waters SAT/IN Module
Bus SAT/IN Module
2410 Differential Refractometer
432 Conductivity Detector
2432 Conductivity Detector
2414 Refractive Index Detector
2420 Evaporative Light Scattering Detector
2424 Evaporative Light Scattering Detector
2465 Electrochemical Detector
2475 Multi-Wavelength Fluorescence Detector
2487 Dual-Wavelength Absorbance Detector
2488 Multichannel Absorbance Detector
2489 UV/Vis Detector
2996 PDA Detector
2998 Photodiode Array Detector
ACQUITY UPLC Tunable UV Detector
ACQUITY UPLC PDA Detector
ACQUITY UPLC PDA eλ Detector
ACQUITY UPLC FLR Detector
ACQUITY UPLC ELS Detector
ACQUITY QDa Mass Detector
CTC PAL Autosampler
Agilent 1260 Infinity
Refractive Index Detector
Agilent 7890 GC
Agilent 7697A Headspace
Agilent 6890 GC
Agilent 6850 GC
AutoSpec Premier
GCT
GCT Premier
Quattro Micro GC
Atmospheric Pressure GC
Quattro Premier
Quattro Premier XE
Quattro LC
Quattro LCZ
Quattro micro API
Quattro Ultima
3100 Mass Detector
ACQUITY SQD system
SQ Detector
ACQUITY TQD system
TQ Detector
SQ Detector 2
Xevo TQ MS
Xevo TQD
Xevo TQ-GC
Xevo TQ-S
Xevo TQ-S cronos
Xevo TQ-S micro
Xevo TQ-XS
ZQ 2000
ZQ 4000
ZMD

CAUSE

Old or wrong chemistry

FIX or WORKAROUND

Use a freshly prepared sample.
Use a fresh column.
Use a fresh precolumn / filter.
Use freshly prepared mobile phases and wash solutions.
Use the correct Method and solvents.

ADDITIONAL INFORMATION

Always double-check; never trust labels.