Low sensitivity or no peaks at certain points - WKB101611
SYMPTOMS
- Low sensitivity
- No peaks
- Incorrect retention time
- Strange baseline
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- 2695D Dissolution Separations Module
- 2795 Separations Module
- Alliance Series Column Heater
- Alliance Series Column Heater/Cooler
- ACQUITY APC
- ACQUITY APC Isocratic Solvent Manager
- ACQUITY APC Sample Manager - FTN
- ACQUITY Arc
- ACQUITY Arc Sample Manager FTN-R
- ACQUITY UPLC
- ACQUITY UPLC BSM (example, use other modules as needed)
- ACQUITY UPLC Column Manager
- ACQUITY UPLC H-Class
- ACQUITY UPLC H-Class Bio
- ACQUITY UPLC H-Class Quaternary Solvent Manager (QSM)
- ACQUITY UPLC I-Class
- ACQUITY UPLC H-Class PLUS
- ACQUITY UPLC H-Class PLUS Bio
- ACQUITY UPLC I-Class PLUS
- ACQUITY UPLC M-Class
- nanoACQUITY UPLC
- PATROL UPLC Process Analysis System
- ACQUITY UPLC Process Sample Manager
- ACQUITY UPLC Column Heater
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC Sample Manager
- ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
- ACQUITY UPLC Sample Organizer
- ACQUITY UPLC Tunable UV Detector
- ACQUITY UPLC FLR Detector
- ACQUITY UPLC RI Detector
- ACQUITY UPLC Online SPE Man
- 510 HPLC pump
- 515 HPLC Pump
- 600 MultiSolvent Delivery Pump
- 717plus Autosampler
- 1500 Series Column Heater
- 1515 Isocratic HPLC Pump
- 1525 Binary HPLC Pump
- 1525 Micro Binary HPLC Pump
- 2707 Sample Manager
- 2777 Sample Manager
- e-SAT/IN Module
- Waters SAT/IN Module
- Bus SAT/IN Module
- 2410 Differential Refractometer
- 432 Conductivity Detector
- 2432 Conductivity Detector
- 2414 Refractive Index Detector
- 2420 Evaporative Light Scattering Detector
- 2424 Evaporative Light Scattering Detector
- 2465 Electrochemical Detector
- 2475 Multi-Wavelength Fluorescence Detector
- 2487 Dual-Wavelength Absorbance Detector
- 2488 Multichannel Absorbance Detector
- 2489 UV/Vis Detector
- 2996 PDA Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC Tunable UV Detector
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC PDA eλ Detector
- ACQUITY UPLC FLR Detector
- ACQUITY UPLC ELS Detector
- ACQUITY QDa Mass Detector
- CTC PAL Autosampler
- Agilent 1260 Infinity
Refractive Index Detector - Agilent 7890 GC
- Agilent 7697A Headspace
- Agilent 6890 GC
- Agilent 6850 GC
- AutoSpec Premier
- GCT
- GCT Premier
- Quattro Micro GC
- Atmospheric Pressure GC
- Quattro Premier
- Quattro Premier XE
- Quattro LC
- Quattro LCZ
- Quattro micro API
- Quattro Ultima
- 3100 Mass Detector
- ACQUITY SQD system
- SQ Detector
- ACQUITY TQD system
- TQ Detector
- SQ Detector 2
- Xevo TQ MS
- Xevo TQD
- Xevo TQ-GC
- Xevo TQ-S
- Xevo TQ-S cronos
- Xevo TQ-S micro
- Xevo TQ-XS
- ZQ 2000
- ZQ 4000
- ZMD
CAUSE
Old or wrong chemistry
FIX or WORKAROUND
- Use a freshly prepared sample.
- Use a fresh column.
- Use a fresh precolumn / filter.
- Use freshly prepared mobile phases and wash solutions.
- Use the correct Method and solvents.
ADDITIONAL INFORMATION
Always double-check; never trust labels.
id101611, 2410, 2414, 2414B, 2420, 2424, 2424B, 2487, 2489, 2489B, 2489SFC, 2690, 2690D, 2690DUPGD, 2690UP, 2695, 2695D, 2695DE, 2695DEB, 2695DUP, 2695E, 2695EB, 2695INERT, 2695UP, 2777, 2777C, 2777IVD, 515, 717, 717HC, 717PLUS, 717UP, A-10RI, A-10SSM, A-10UV, A-30MCM, A-30PDA, A-30RI, A-30SM, A-30UV, A-SQ, A-SQ, eluent, PTROLOPSM, Q3100MSD, QDA, SQD, SQD2, UPACM30S, UPACMS, UPAPDA, UPARPTUV, UPASMFTN, UPBINARY, UPBSM+, UPBSMIVD, UPC2BSM, UPC2CM30S, UPC2CMA, UPC2CMAUX, UPC2CNVMGR, UPC2PDA, UPC2SMFL, UPCMA, UPCMAIVD, UPCMAUX, UPCMIVD, UPCOLMGR, UPELS, UPELSARC, UPIBSM, UPIBSM, UPIBSM+, UPIBSM+, UPIBSMIVD, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN, UPISMFTN+, UPLCCHIVD, UPLCCOLHTR, UPMASM, UPMBSM, UPMSMFL, UPNAYXPMP, UPNBINARY, UPNSAMMGR, UPNSOLMGR, UPPBSM, UPPCMA, UPPCMAUX, UPPDA, UPPDAARC, UPPDAARCB, UPPDA-E, UPPDA-L, UPPDALTC, UPPDATC, UPPFTN, UPPPDA, UPPTUV, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPRI, UPRIARC, UPSMFTN, UPSMFTN, UPSMFTN+, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR, UPSMPMGR, UPTUV, UPTUVARC, UPTUVARCB, UPTUV-E, UPTUVTC, XEVOG2QTOF, XEVOG2SQTF, XEVOG2STOF, XEVOG2TOF, XEVOG2XSQT, XEVOG2XSTF, XEVOQTOF, XEVOTQ, XEVOTQA, XEVOTQD, XEVOTQDIVD, XEVOTQGC, XEVOTQIVD, XEVOTQS, XEVOTQSCRO, XEVOTQSIVD, XEVOTQSMIC, XEVOTQXS, XEVTQSMIVD, XEVTQXSIVD, ZQ2000, ZQ2000SFC, ZQ4000