"Sample fluidics high pressure limit (value) error" with ACQUITY UPLC Sample Manager-FL - WKB100139

SYMPTOMS

• "Sample fluidics high pressure limit (value) error"
• Replacing the sample needle does not solve the problem

ENVIRONMENT

• ACQUITY UPLC Sample Manager 

CAUSE

Clog in the injection valve cartridge

FIX or WORKAROUND

Step one is to try to flush away the clog, as follows:

1. Remove lines from ports 2 and 3.
2. Take the line from port 5 and put it in port 3, and set flow to 0.1 mL/min for about two minutes.
3. Take the line from port 5 and put it in port 2, and set flow to 0.1 mL/min for about two minutes.
4. Put all lines back to their respective ports.

5. Prime the syringes.

6. Verify that clog has been flushed away.

If clog persists, and it has been located in the injection valve:

1. Replace the injection valve cartridge (700002765).

ADDITIONAL INFORMATION

Instructions on replacing the injection valve cartridge are in the ACQUITY UPLC System Operator's Guide (71500082502).