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Waters

In UNIFI, an unwanted mass shows up in the spectra - WKB28557

Article number: 28557

SYMPTOMS

  • Processed MSe data on the Review tab shows a mass in the low and high energy spectra that belongs to another (slightly overlapping) peak
  • Doing an extracted mass chromatogram shows that the mass is associated with the other peak in the Investigate tab
  • Changing the Processing > Targeted Screen Settings > Target by Retention Time > Identify Target Peak using rule: Largest in the RT window to that of Best in the RT window (based on identified fragments etc.) does not change the results
  • Changing the Peak Processing > Find 2D peaks > Peak Detection threshold from a set Detection threshold to Automatic Detection does not change the results
  • Intensity of the data is at 4e7 (at 0.3 second scans)

ENVIRONMENT

  • UNIFI

CAUSE

The peak processing settings for 3D Isotope are incorrect.

FIX or WORKAROUND

  1. Open the method.
  2. Browse to the Processing section.
  3. Go to Peak Processing Settings and for 3D Isotope select Clustering.
  4. Clear the option "Allow wider Chromatographic Tolerance for Saturated Data".3D isotope.png
  5. Update the method and use.

ADDITIONAL INFORMATION

 

SUPUNIFI, UNIFISVR, UNIFISW18, UNIFOPT, UNIFQLIC, UNIFSW17, UNIFSW18, UNIFSW19, UNIFWGLIC, UNIFWKLIC

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