In UNIFI, is it possible to separately identify two homologous peptides that are part of the same protein sequence? - WKB100011
Article number: 100011
ENVIRONMENT
- UNIFI (all versions)
ANSWER
UNIFI is not able to separately identify peptides of same sequence within a protein sequence. Especially if they co-elute, there is always only one observed mass and therefore one identification. (The other IDs are in alternate assignments, but even if you change the assignment it is only one at the time.) Therefore, it is not possible to obtain 100% protein coverage.
ADDITIONAL INFORMATION
id100011, SUPUNIFI, UNIFISVR, UNIFISW18, UNIFOPT, UNIFQLIC, UNIFSW17, UNIFSW18, UNIFSW19, UNIFWGLIC, UNIFWKLIC