QDa MS Data in Empower - Smoothing Chromatograms - Tip358

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Article number: 276039

OBJECTIVE or GOAL

Smoothing Chromatograms

ENVIRONMENT

Empower
Empower Tip of the Week #358

PROCEDURE

STEP 1
Without any smoothing, we see the signal-to-noise ratio of our peak of interest is 29:1 (figure 1).

STEP 2
Go to the Results window within Review. We see the height, and signal-to-noise ratio in the Peaks table and the peak-to-peak noise in the Chromatogram Result table, which is 33659 (figure 2).

STEP 3
Go to the Smoothing/Offset tab of the Processing Method. We can apply a small amount of smoothing, in this example a smoothing level of 9 (figure 3).

STEP 4
Reprocessing the data we see the signal-to-noise ratio is now 42.5:1 (figure 4).

STEP 5
Returning to the Results window within Review, we see about a 1% reduction in peak height and a significant reduction in peak-to-peak noise which is now 22715, resulting in an improvement in the signal-to-noise ratio (figure 5).

ADDITIONAL INFORMATION

Savitzky-Golay smoothing averages data points. For more detailed information Google a book called Chromatographic Integration Methods by Norman Allen Dyson. There is a good section on filtering and smoothing chromatographic signals.