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How to use the average area of replicate injections of a sample to quantitate analyte(s) of interest (Part 16)

Article number: 56954

OBJECTIVE or GOAL

Welcome back to Get Empowered! In the last Empower tip-of-the-week post for Empower Chromatography Data Software, we learned how to report the maximum pressure during a chromatographic run (Tip #97).

Creating Custom Fields in Empower:

  • #82: The basic steps on creating a Custom Field
  • #83: How to create a Custom Sample Set Field
  • #84: How to create a Component Custom Field
  • #85: How to create a Peak Custom Field
  • #86: How to copy a Custom Field from one project to another
  • #87: How to modify a Custom Field
  • #88: How to create a Result Custom Field
  • #89: How to modify a Custom Field that applies to specific samples and/or specific peaks
  • #90: How to use Empower to evaluate whether the sample meets specific criteria
  • #92: How to create one custom field to determine a sample pass/fail
  • #93: How to create an Intersample Custom Field
  • #94: How to create Intersample Custom Fields with a summary function
  • #95: The difference between Summarize Custom Fields and Summarize Custom Fields Incrementally
  • #96: How to report the pressure and temperature at a certain time point within a chromatogram
  • #97: How to report the maximum pressure during a chromatographic run

This week, I want to share another great question I received.

Q: How do I use the average area of replicate injections of my sample to quantitate my analyte(s) of interest?

A: Normally, we quantitate the individual injections and then calculate the average amount; this is another way.

In this tip, I will show you how to use the average area of replicate injections of a sample to quantitate analyte(s) of interest (Part 16).

Let’s get started.

ENVIRONMENT

  • Empower

PROCEDURE

  1. Create a Peak Type Custom Field, ‘data type real’ to calculate the average area of the replicate injections. This is an Intersample Custom Field with a summary function (figure 1).

    56954-01.png

    Figure 1

  2. The equation for a line is y=mx+b. In Empower, it is y=Bx+A. If we solve for ‘x’, we get x=(y-A)/B. In this case, we want to use the ‘average area’ which is calculated from the first Custom Field. I would recommend referencing the ‘last injection’ of the ‘last standard’ for A and B, so that you have the final coefficients (figure 2).

    56954-02.png

    Figure 2

  3. Now, we see the ‘average area’ for each component based on the duplicate injections of the sample and the ‘calculated amount’ based on that average (figure 3).

    56954-03.png

    Figure 3

It’s that easy!

ADDITIONAL INFORMATION

Final notes:

  • This procedure can be followed using the QuickStart or Pro interface.
  • Please refer to Tip #93 and Tip #94 for more information about Intersample Custom Fields.

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