How to reuse an older calibration curve in Empower - WKB274031

OBJECTIVE or GOAL

Reuse an older calibration curve to quantitate samples. (Every time Standards are calibrated, Empower assigns a Calibration ID to the calibration curves
generated. If you want to use a calibration curve other than the current one, you need to select the older curve using the Calibration ID number.)

ENVIRONMENT

• Empower

PROCEDURE

1. In this example, the current calibration has ID 6240 and the older calibration has ID 6213. First look at the difference between them using the Compare window.

2. In Compare Window with Calibration ID 6240 highlighted, click the Individual Points tab.

3. The X Values for the three levels are 0.02, 0.05, and 0.1, respectively.

4. The older Calibration ID of 6213 has X Values that are slightly lower due to a Purity factor that was applied. The goal is to use this older calibration curve where the Purity factor had been applied.

5. Bring a Result Set or a Result into Review and click the Calibration Curve tool.

 

6. In the Calibration Curve window, go to Options > Select Calibration.

7. Select the Calibration Curve you want to use (in this case, Calibration ID 6213) and click OK.

8. Click OK to the warning message that says the calibration you have selected is not the most current.

9. Calibration curves are associated with the processing method, so from the File menu select Save As > Processing Method to give the method a different name.

10. You will be asked to Clear Curves or Copy Curves. Select Copy Curves.

11. Specify a new name for the Processing Method and click Save.

12. Exit from Review and process the Sample Set using the older calibration curve. Go to the Sample Sets tab in Browse Project, right-click on the Sample Set, and select Process.

13. In the Background Processing and Reporting dialog box, select the Processing Method with the different name, and from the How drop-down menu, select Quantitate Only. Then click OK.

14. Go to the Result Sets tab, right-click on the new Result Set, and select Review.

 

15. Click the Calibration Curve tool.

16. As seen in our example, the X Values for Uracil are from the older calibration curve.

17. If we compare the results for a sample, we see a different calculated amount for Uracil when we use the older calibration curve.

ADDITIONAL INFORMATION