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Re-using older calibration curve in Empower - WKB274031

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Article number: 274031

OBJECTIVE or GOAL

How to use an older calibration curve to quantitate samples. Every time Standards are calibrated, Empower assigns a Calibration ID to the calibration curves
generated. If one wants to use a calibration curve other than the current one, one need to select the older curve using the Calibration ID number.

ENVIRONMENT

  • Empower

PROCEDURE

1.       In this example, the current calibration has ID 6240 and the older calibration has ID 6213. First look at the difference between them using the Compare window.

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2.       In Compare Window with Calibration ID 6240 highlighted, click the Individual Points tab

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3.       The X Values for the three levels are 0.02, 0.05, and 0.1 respectively

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4.       The older Calibration ID of 6213 has X Values that are slightly lower due to a Purity factor that was applied. The goal is to use this older calibration curve where the Purity factor had been applied.

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5.       Bring a Result Set or a Result into Review and click the Calibration Curve tool

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6.       In the Calibration Curve window go to Options, Select Calibration

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7.       Select the Calibration Curve you want to use (in this case Calibration ID 6213) and click OK

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8.       Click OK to the warning message that says the calibration you have selected is not the most current

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9.       Calibration curves are associated with the processing method, so from the File menu select Save As, Processing Method to give the method a different name

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10.   You will be asked to Clear Curves or Copy Curves. Select Copy Curves

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11.   Enter a new name for the Processing Method and click Save

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12.   Exit from Review and now process the Sample Set using the older calibration curve. Go to the Sample Sets tab in Browse Project, right-click on the Sample Set and select Process

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13.   In the Background Processing and Reporting dialogue box select the Processing Method with the different name, and from the How drop-down menu select Quantitate Only. Then click OK.

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14.   Go to the Result Sets tab, right-click on the new Result Set and select Review.

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15.   Click the Calibration Curve tool.

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16.   As seen in our example, the X Values for Uracil are from the older calibration curve.

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17.   If we compare the results for a sample, we see a different calculated amount for Uracil when we use the older calibration curve.

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ADDITIONAL INFORMATION

 

id274031, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

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