How to create an FLR method for unknown compounds (excitation wavelength and fluorescence wavelength unknown, UV absorption spectrum known) - WKB117617
Article number: 117617
OBJECTIVE or GOAL
Create a measurement method for unknown compounds (excitation wavelength and fluorescence wavelength unknown, UV absorption spectrum known).
ENVIRONMENT
- ACQUITY UPLC FLR Detector
- Empower 3
PROCEDURE
1. In 3D mode, fix the excitation wavelength at a wavelength with high UV absorption, and scan the fluorescence wavelength.
2. Confirm the optimum value of fluorescence wavelength from the data in step 1.
3. Specify the fluorescence wavelength confirmed in step 2 in 2D mode and measure.
ADDITIONAL INFORMATION
id117617, A-30FL, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP, UPFLRARC, UPFLRDET, UPPFLR