Are the proteins from my plasma eluting in the 100 percent aqueous loading conditions on a reverse phase SPE sorbent bed? - WKB96402
Article number: 96402
ENVIRONMENT
- SPE
- Solid Phase Extraction
- protein
- plasma
- reverse phase
- Oasis HLB
- Oasis PRIME HLB
- Sep-Pak C18
ANSWER
No. Pretreated plasma samples in which high salt, organic, or acid are used to denature the proteins, result in altered protein structures that are preferentially more nonpolar.
ADDITIONAL INFORMATION
For additional information, see the Sample Pre-Treatment section of the Oasis Reference Cards.
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