Can I spin down 96-well plates during GlycoWorks RFMS, instead of using positive/negative pressure? - WKB85264
Article number: 85264
ENVIRONMENT
- HILIC
- 96-well collection plates
- GlycoWorks RFMS
ANSWER
Yes, you can. Centrifugation provides a consistent force that, because it ramps up slowly, usually leads to a good flow rate and complete elution. It typically forces a "complete elution" from the sorbent bed, helping to overcome losses due to lack of vacuum or pressure.
Waters recommends that you centrifuge the collection plate at 4,000 g to get a flow rate of about 30-50 μL/min.
ADDITIONAL INFORMATION
id85264,