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Waters

Why is it necessary to have 20 percent water present in the wash and elution steps of an SPE protocol for peptides? - WKB68486

Article number: 68486

ENVIRONMENT

  • Solid-phase extraction
  • SPE
  • peptide
  • protocol
  • Oasis
  • Sep-Pak

ANSWER

Approximately 20% water is recommended to prevent peptide precipitation. Precipitation of peptides affects sample recovery.

ADDITIONAL INFORMATION

For additional information, see the Oasis Peptide Bioanalysis Reference Card.

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