Why is it necessary to have 20 percent water present in the wash and elution steps of an SPE protocol for peptides? - WKB68486
Article number: 68486
ENVIRONMENT
- Solid-phase extraction
- SPE
- peptide
- protocol
- Oasis
- Sep-Pak
ANSWER
Approximately 20% water is recommended to prevent peptide precipitation. Precipitation of peptides affects sample recovery.
ADDITIONAL INFORMATION
For additional information, see the Oasis Peptide Bioanalysis Reference Card.
id68486,