- GlycoWorks RapiFluor-MS
- Glycan labeling
- Sulfated glycans
RapiFluor-MS works well with glycans that have a sulphate group.
A neutral loss of 80 Da indicates the loss of a SO3 group upon MS/MS fragmentation. SimGlycan can annotate the fragment ions.
This work can be referenced in the application note Analysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS. The work in this paper used the 2AB label, but it has been proven that 2AB and RFMS tagged glycans show similar fragmentation pathways up CID; therefore, there is high confidence that RFMS labeled sulphate containing glycans will give you the -80 Da fragment ions.