How do I desalt oligonucleotides offline? - WKB59559

ENVIRONMENT

• Solid-phase extraction (SPE)
• Oasis
• Hydrophilic-Lipophilic balance (HLB)
• Oligonucleotide
• Oligo
• Desalting
• uElution Plate

ANSWER

See Desalting of DNA Oligonucleotides by SPE for MALDI Analysis

ADDITIONAL INFORMATION

The application note above refers to desalting of "DMT-off" (Trityl off, deprotected) oligonucleotides by using an ion-pair, reversed phase technique.

An additional application note focuses on desalting of "DMT-on" (Trityl on, protected) oligonucleotides:

DMT On Purification of DNA Oligonucleotides <35mer Using Oasis HLB SPE Products [Excerpt from Oasis Applications Notebook]   (lit code WA31764.71)