Do I need to condition and equilibrate Oasis solid-phase extraction plates and cartridges prior to loading sample? - WKB57060
Article number: 57060
ENVIRONMENT
- Oasis SPE products
- Solid-phase extraction (SPE)
- Sample preparation
- Oasis PRIME HLB
- condition and equilibrate
ANSWER
No, depending on the analytes/application
ADDITIONAL INFORMATION
Waters has found that, because Oasis solid-phase extraction packings are water wettable, it may not be necessary to perform the conditioning and equilibration steps prior to loading aqueous samples.
It is possible to directly load aqueous samples and then proceed to any wash steps and the final elution step without performing any conditioning or equilibration steps.
In order to achieve optimal analyte recoveries, it is advisable to experiment both with and without conditioning and equilibration to confirm that a particular application does not require these two steps prior to sample load. .
Oasis® Cartridges and 96-Well Plates Care & Use Manual
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