Why do my GlycoWorks RapiFluor-MS labeled N-Glycan peaks show a lower area count when I have histidine in my samples? - WKB53577

ENVIRONMENT

• Glycan analysis
• GlycoWorks RapiFluor-MS

ANSWER

Histidine contains nucleophiles, which compromise rapid labeling with RapiFluor-MS.

ADDITIONAL INFORMATION

The presence of histidine in a sample consumes a substantial amount of the RapiFluor-MS reagent before the reagent fully reacts with the N-Glycans present in a sample. It is advised that you dilute amine or thiol concentrations in a sample to less than 0.1 mM in order to alleviate this type of issue. Some samples may require a buffer exchange prior to enzymatic deglycosylation in the GlycoWorks RapiFluor-MS workflow.

See: 

GlycoWorks RapiFluor-MS N-Glycan Kit - 96 Samples

GlycoWorks RapiFluor-MS N-Glycan Kit - 24 Samples Care and Use Manual