Can I use methanol as a precipitation solvent for the Sirocco Protein Precipitation plate? - WKB267034
Article number: 267034
ENVIRONMENT
- Sirocco
- 96-well plate format
- Plasma protein precipitation
- Solvent
- Methanol
ANSWER
Methanol is not the recommended crash solvent to use with the Sirocco Protein Precipitation plate.
ADDITIONAL INFORMATION
Methanol produces a stringy, viscous plasma precipitation that more easily clogs the Sirocco plate wells.
Acetonitrile produces a precipitation that is more likely to pellet and be less viscous.
For additional information, see What is the standard operating procedure for the Sirocco Protein Precipitation Plate (186002448 or 186003873)?
id267034,