How do I choose among protein crash, LLE, and SPE for a sample preparation technique? - WKB256529

ENVIRONMENT

• Sample preparation
• Solid-phase extraction (SPE)
• Liquid liquid extraction (LLE)

ANSWER

See Three Reasons to use SPE.

ADDITIONAL INFORMATION

Consider how much system downtime is likely if a sample is minimally processed/clarified prior to injection on an LC system. This is sample- and matrix-dependent.

Here are the Waters sample prep techniques from removal of least matrix constituents to most (least is simple filtration and most is Oasis Mixed-Mode):

Simple filtration > protein precipitation > Sirocco protein precipitation plate > Ostro plate > Oasis HLB > Oasis Mixed Mode sorbents like MCX