How can I retain my acidic compound preferentially to the Oasis PRIME HLB sorbent? - WKB232348
Article number: 232348
ENVIRONMENT
- Solid-phase extraction (SPE)
- Reverse phase
- Method development
- Oasis PRIME HLB
ANSWER
Add an acid modifier like 2% formic acid to the load and wash steps. A weakly acidic compound can become more neutralized under acidic conditions and a neutralized ionic compound is more nonpolar, allowing it to bind more easily to the nonpolar sorbent.
ADDITIONAL INFORMATION
For additional information, see What is the retention mechanism of Oasis PRIME HLB solid-phase extraction devices?
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