How much organic solvent can I have in my sample prior to loading onto an Oasis HLB SPE device? - WKB230880

ENVIRONMENT

• Solid-phase extraction (SPE)
• Hydrophilic-lipophilic balance (HLB)
• Sample preparation
• Oasis HLB

ANSWER

This depends on the SPE method used (pass through vs. capture and elute).

ADDITIONAL INFORMATION

If performing a flow-through method (where analyte is not retained, but sample matrix is retained):

• Ideally, use a 100% aqueous sample diluent to prevent breakthrough of unwanted matrix interferences.
• Experimentation is required to determine how much organic can be present in your sample without eluting matrix interferences during the loading step.

If performing a capture and elute method (where analyte retains during the loading step and elutes during the final elution step):

• Ideally, use a 100% aqueous sample diluent to prevent breakthrough of your analyte during the loading step.
• Experimentation is required to determine how much organic can be present in your sample without eluting your analytes of interest during the loading step.