How to clean up peptides on Oasis MCX - WKB22755
Article number: 22755
OBJECTIVE or GOAL
Clean up peptides on an Oasis MCX 225-mg cartridge.
ENVIRONMENT
- SPE
- Oasis MCX
- Peptides
PROCEDURE
- Access 720005685EN on the Waters website and see page 6 for the Oasis 2 x 4 method.
- See page 7 for directions on sample pretreatment with phosphoric acid.
- Before adding sample to the cartridge, condition with MeOH or ACN and equilibrate with water.
- Once the peptide sample is loaded onto the sorbent bed, all washes and elution solvent should contain a minimum of 20% water.
ADDITIONAL INFORMATION
Example of positive-pressure use of a "plus" body style cartridge:
http://www.waters.com/waters/en_US/S...htm?cid=513158
For additional information on using Oasis MCX for peptides, see the Oasis Peptide Bioanalysis Reference Card.
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