OBJECTIVE or GOAL
Clean up peptides on an Oasis MCX 225-mg cartridge.
- Oasis MCX
- Access 720005685EN on the Waters website and see page 6 for the Oasis 2 x 4 method.
- See page 7 for directions on sample pretreatment with phosphoric acid.
- Before adding sample to the cartridge, condition with MeOH or ACN and equilibrate with water.
- Once the peptide sample is loaded onto the sorbent bed, all washes and elution solvent should contain a minimum of 20% water.
Example of positive-pressure use of a "plus" body style cartridge:
For additional information on using Oasis MCX for peptides, see the Oasis Peptide Bioanalysis Reference Card.