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How to clean up peptides on Oasis MCX - WKB22755

Article number: 22755

OBJECTIVE or GOAL

Clean up peptides on an Oasis MCX 225-mg cartridge.

ENVIRONMENT

  • SPE
  • Oasis MCX
  • Peptides

PROCEDURE

  1. Access 720005685EN on the Waters website and see page 6 for the Oasis 2 x 4 method.
  2. See page 7 for directions on sample pretreatment with phosphoric acid.
  3. Before adding sample to the cartridge, condition with MeOH or ACN and equilibrate with water.  
  4. Once the peptide sample is loaded onto the sorbent bed, all washes and elution solvent should contain a minimum of 20% water.

ADDITIONAL INFORMATION

Example of positive-pressure use of a "plus" body style cartridge:

http://www.waters.com/waters/en_US/S...htm?cid=513158

For additional information on using Oasis MCX for peptides, see the Oasis Peptide Bioanalysis Reference Card.

methanol

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