Can GlycoWorks RapiFluor-MS be used to fully label N-released glycans from protein trimers? - WKB204582
Article number: 204582
ENVIRONMENT
- GlycoWorks
- RapiFluor-MS
- Glycans
ANSWER
It is possible.*
ADDITIONAL INFORMATION
A protein trimer may be similar to the SARS-CoV-2 spike protein, which is a ~540 kDa homotrimeric glycoprotein.
Waters has done some studies on the spike protein using a reducing protocol (see the following links) and identified 42 major glycan peaks.
Quick Start Reducing Protocol – 24 samples
Quick Start Reducing Protocol – 96 samples
In the reducing protocol, we use DTT to help digest the molecules with multiple disulfide bonds.
The protocol is optimized for glycoprotein with a concentration of 1.5 mg/mL.
*You can use the reducing protocol as a starting point and monitor the intact mass to determine if the deglycosylation is complete.
id204582,