Can I use solid-phase extraction (SPE) to extract peptides away from intact proteins in the same sample? - WKB123970
Article number: 123970
ENVIRONMENT
- Hydrophilic-lipophilic balance (HLB)
- Solid-phase extraction (SPE)
- Sample cleanup
- Peptide extraction
- Oasis
ANSWER
Yes
ADDITIONAL INFORMATION
Oasis HLB is recommended because it has 80Å pores, which will exclude most proteins from entering.
Peptides are small enough to enter the pores in this packing.
Make sure to load sample slowly so that the peptides have enough time to enter the pores and retain.
Final elution should be performed with a slow flow rate so that the peptides have enough interaction with the elution solvent.
The loading and elution steps should result in individual droplets exiting the bottom of the SPE device.
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