Can I use the AccQ•Tag Ultra Derivatization Reagent to analyze free amino acids in the presence of intact proteins? - WKB123834
Article number: 123834
ENVIRONMENT
- AccQ•Tag Ultra
- Amino acid analysis (AAA)
- Intact protein
- Free amino acids
- Derivatization
ANSWER
No
ADDITIONAL INFORMATION
AccQ•Tag Ultra Derivatization Reagent will derivatize any primary and secondary amines present in a sample. If proteins are present along with the amino acids of interest, the proteins will consume the majority of the reagent, which will result in incomplete or non-reproducible derivatization of the free amino acids of interest.
Proteins should be removed from the sample matrix prior to derivatizing the free amino acids of interest.