Is it better to use 0.04% TFA or 0.1% TFA in the mobile phase for peptides? - WKB89930
Article number: 89930
ENVIRONMENT
- Peptide analysis
- Mobile phase
- Trifluoroacetic acid (TFA)
ANSWER
The appropriate level of TFA in mobile phase is method-specific, influenced by the peptide solubility, tailing, columns, and the mobile phase and detectors used.
ADDITIONAL INFORMATION
Peptide solubility and chromatographic tailing is influenced by the type and concentration of the acid modifier in the sample diluent and mobile phase.
id89930, eluent