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How do I confirm that my flow rate matches the rate set in the LC method? - WKB32146

Article number: 32146

OBJECTIVE or GOAL

Confirm that the delivery flow rate matches what is stated in the method, in order to identify the cause of longer retention times in the chromatography.

ENVIRONMENT

  • HPLC
  • UPLC

PROCEDURE

  1. In a volumetric flask, measure the volume of liquid eluting from the outlet of the column at the set flow rate for a given period of time.
  2. For example, if the flow rate is set to 1 mL/min,  when the volumetric flask is full, it should use approximately 10 min

ADDITIONAL INFORMATION

 

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