What is the difference between AccQ•Tag Eluent A parts 186003838 and WAT052890? - WKB28284

ENVIRONMENT

• Amino acid analysis
• AccQ•Fluor
• AccQ•Tag Ultra
• ACQUITY UPLC
• HPLC
• Eluent
• SDS

ANSWER

186003838 was formulated to run for amino acids derivatized with AccQ•Tag Ultra reagent and for analysis of hydrolysates and cell culture samples using ACQUITY UPLC Classic and ACQUITY UPLC H-Class and H-Class Bio. It contains water, acetonitrile, and approximately 3-7% formic acid. It is a volatile mobile phase.

WAT052890 is an older formulation for running amino acids derivatized with AccQ•Fluor reagent and for analysis of hydrolysates and cell culture samples using an HPLC system, primarily with a fluorescence detector and secondarily with a UV detector. Upon normal dilution per the HPLC instructions, it contains approximately 125 mM salt-based mobile phase; it is not volatile. When used mistakenly as a substitute for the ACQUITY UPLC amino acid solution, the selectivity of the amino acids is very different, but an even more immediate problem is precipitation of the salt inside the flow path. This is NOT THE CORRECT eluent to use with the ACQUITY UPLC amino acid systems.

ADDITIONAL INFORMATION

For additional information, see the safety data sheets at www.waters.com/sds