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Will the HPLC UV detector allow me to measure the concentration of my sample with UV detection? - WKB246447

Article number: 246447

ENVIRONMENT

  • Liquid chromatography
  • HPLC
  • UPLC
  • UV detector
  • Sample concentration

ANSWER

Yes, you can measure the concentration of a compound with an HPLC UV detector running a chromatographic separation. 

ADDITIONAL INFORMATION

To be measured, the compound must have a chromaphore in order. The chromaphore could be in the nature of the structure of the compound, or added to a compound by labeling with a UV-absorbant derivatization reagent or "tag".

For additional information, see the Beginner's Guide to Liquid Chromatography.

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