What causes early eluting peaks and tailing when setting up the AccQ•Tag Ultra turnkey solution on a H-Class? - WKB243721

ENVIRONMENT

• Amino acid analysis (AAA)
• ACQUITY H-Class
• AccQ•Tag Ultra
• Peak tailing
• Early eluting peaks

ANSWER

The needle purge wash gets injected with the sample and should be changed to 90:10 water/acetonitrile.

ADDITIONAL INFORMATION

The early eluting peaks are affected by strong solvent effect when the percentage of organic is higher than the starting conditions of the mobile phase.

For additional information, see the impact on peak shape in the evaluation of the wash solutions included in the following applications note:

Instrument Considerations for Successful Adaptation of Amino Acid Analysis Methods Which Utilize Pre-Column Derivatization From an ACQUITY UPLC to an ACQUITY UPLC H-Class PLUS Binary System