Am I running 1D chromatography on a nano system if I have set up a single pump trap and elute flow path? - WKB221190

ENVIRONMENT

• 1D chromatography
• nanoACQUITY
• ACQUITY M-Class

ANSWER

The answer is "maybe".  "1D" is used to describe the chemistry used and, sometimes, the flow path used.

ADDITIONAL INFORMATION

The single pump trap and elute path using a reverse-phase trap and a reverse-phase column would be called “1D”, as in the following application note example:

Sub-Femtomole Detection of Protein Digests Using Q-Tof and nanoACQUITY UPLC system

Direct flow into a reverse-phase nano column is also described as “1D”, as in the following application note example:

A Comprehensive Approach for HCP Identification

The single pump trap and elute path using an SCX (strong cation exchange) trap and a reverse-phase column would be using the “1D” flow path but the “2D” chemistry/separation (first-dimension ion exchange, second-dimension reverse phase), so it would be called “2D” chromatography.